Esan-Gene696/696A Real-time PCR System

Real-time PCR System — Esan-Gene 696/696A
Esan-Gene 696/696A real-time PCR system adopts mature thermoelectric refrigeration technology, brand-new light source and light path design. The unique constant-current power and multi-zone independent temperature control method ensure more rapid, correct and stable fluorescence quantitative analysis of the product, while maintaining its excellent performance in low energy consumption. The product is modularized with various configurations to choose from. Meanwhile, it has been added with functions including independent temperature control, low-temperature storage of sample at 4 °C, and automatic dehumidification.

It’s mainly applied in gene expression, genotyping (detection), cloning, mutation, methylation analysis, sequencing, medicine, forensic medicine and applied science.

CMOS Imaging Photoelectric Detection
a. Top imaging technology was adopted to collect 96-well fluorescent signals without detection time difference. Fast detection, single channel detection need only 1 second.
b. Long-life LED light source: adopting maintenance-free long-life LED light source, no need to replace the light source regularly, and the light intensity distribution is uniform.
c. The excitation and detection channels adopt independent filter wheels. The filter can be switched quickly, and the channels can be freely combined, which can cope with multiple detection experiments without expanding the channel.
d. Using optical fiber conduction to improve the fluorescence signal strength, reduce the optical conduction loss, and eliminate the edge effect in the optical path.

Precise Temperature Control Technology
a. The multi-zone thermal cycle block has precise independent temperature control and unique block temperature compensation technology. It can set different reaction conditions, with fast reaction speed, high temperature uniformity, and fast heating speed.
b. The thermo lid is adjustable with high sealing to prevent reagent volatilization.
c. Equipped with consumable placement inspection function, automatic pop-up sample warehouse design, and unique anti-pinch design, improving the safety of use.

Humanized Software
a. Real-time monitoring, automatic distinguishing and calculation of positive and negative results, meeting various analysis modes such as qualitative / quantitative, genotyping, isothermal amplification, melting curve, etc.
b. 10.1-inch touch screen with Windows system, can complete settings, detection, analysis, saving and other operations. The operation is simple and fast, without the need to connect to PC, saving space. Equipped with USB and network port for easy data export and greater flexibility.
c. The innovative Ct value algorithm uses the linear fitting method of multi-derivative feature points to calculate the Ct value without the need for reference dyes, optimizing the experimental process and improving the experimental efficiency.
d. Unique optical path ROI region identification algorithm, can achieve automatic instrument ROI calibration and optical fiber position inspection.

Spec:

Model Esan-Gene 696/696A
Applicable centrifuge tube (Esan-Gene 496 / 696) 0.2 mL 96-well plate, single tube, 8-strip tube
Applicable centrifuge tube (Esan-Gene 496A / 696A) 0.1 mL 96-well plate, single tube, 8-strip tube
Sample system size 10~100 μL
Channel number 6-channel
Channel parameter F1: FAM, SYBR Green I, etc. F2: HEX, JOE, VIC, etc. F3: ROX, TEXAS-RED F4: Cy5, Quasar-670 F5: Cy5.5 F6: Can be customized, default is FRET
Linear range 10^0~10^10 Copies/μL
Minimum detection range 1 Copy
Excitation light source High power white LED
Detecting element High-performance CMOS
Ct repeatability CV ≤1 %
Sample linearity (Ct) |r| ≥0.999
Temp. range 4~99.9 °C
Temp. control mode Block control (Block) / analog tube control (Tube)
Average heating rate 4.5 °C/s
Average cooling rate 2.5 °C/s
Temp. uniformity ±0.2 °C @95 °C
Temp. accuracy ±0.15 °C
Temp. control precision 0.1 °C
Melting temp. Minimum differentiation 0.1 °C
Gradient mode 6 gradient temperature control
Gradient temp. range 1~40 °C
Thermo lid temp. 30~110 °C
Display screen 10.1-inch touch screen
Dimension (W*D*H) 298 mm×530 mm×430 mm
Input power 100~240 VAC 50~60 Hz
External port USB (Type A)×3, LAN ×1
Wireless function Expandable wireless transmission function, support LIS function
Data export format TXT, Excel, CSV, PDF

 

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