BPN-40RHP(T)/BPN-60RWP(T) Series CO2 Incubator

Touchscreen Controller

  • A large touchscreen replaces conventional buttons for simple operation and easy program editing. Chinese and English controller interfaces are selectable;
  • Displays real-time operating curves and provides curve summaries. Temperature, humidity and CO2 concentration curves for the same period can be viewed directly, together with abnormal alarms and door-opening/closing information;
  • After data and test conditions are entered, the controller’s screen-lock function prevents accidental touching from causing unintended shutdown;
  • A 72-hour operating-record query function facilitates tracking of abnormal conditions and review of operating history;
  • An optional RS-485 communication interface allows programs to be designed on a computer, experiments to be monitored, and start-up, shutdown and other functions to be performed;

CO2 Concentration Control System

  • Imported infrared (IR) sensor control: an infrared sensor is the best choice when the door must be opened frequently during experiments. The imported IR sensor selected by Yiheng is highly sensitive to changes in CO2 concentration, provides high measurement precision, and is unaffected by other conditions inside the incubator. It avoids the effects of chamber temperature and humidity on conventional thermal-conductivity probes during CO2 monitoring. After the door is opened for 30 seconds and then closed, recovery takes ≤3 minutes to reach the set CO2 concentration of 5%. Even with multiple users and frequent door opening and closing, the chamber CO2 concentration remains rapidly stabilized and uniform.

O2 Concentration Control System (Optional)

  • A zirconia O2 concentration sensor offers good linearity and long service life, providing precise, stable O2 concentration control;
  • The O2 concentration control range is 1~90% (minimum 0.1%), enabling one tri-gas incubator to support hyperoxic, hypoxic and microaerophilic culture;

Temperature Control / Monitoring System

A. Chamber Temperature Control System

  • A Pt100 temperature sensor ensures accurate chamber temperature. Heating power is adjusted in real time according to the difference between the actual and set temperatures, ensuring accuracy of the actual chamber temperature. The experimental temperature can be restored within 3 minutes after the door is opened and closed to remove or load samples;

B. Door Heating System

  • The outer door is heated, with its temperature tracking slightly above the chamber temperature. This effectively prevents condensation on the glass door, facilitates observation of experiments and avoids potential microbial contamination caused by condensation;

C. Ambient Temperature Monitoring System

  • An independent ambient temperature detector automatically adjusts the CO2 incubator heating system in response to changes in ambient temperature during experiments, preventing chamber temperature overshoot;

D. Overtemperature Protection System

  • A backup temperature-control system operates independently of the CO2 incubator chamber temperature-control system. If a fault causes loss of temperature control and the chamber reaches the controller’s temperature-limit setting, the overtemperature protection system automatically cuts off heating and activates audible and visual alarms;

E. Power Failure Alarm System

  • The incubator power supply is monitored in real time. An interruption or loss of power immediately triggers audible and visual alarms, ensuring safe, reliable operation without accidents;

Sterilization System

A. 90°C High-temperature Moist-heat Sterilization System (RHP)

  • High-temperature moist-heat sterilization of the chamber, including the temperature sensor, CO2 concentration sensor, fan, shelves and supports, eliminates microbial contamination of cell cultures caused by bacteria, mold, mycoplasma and other microorganisms, providing a safe experimental environment;
  • Simple operation: press the Sterilization Start button on the control panel to sterilize all chamber components, including the temperature sensor, CO2 concentration sensor, fan, shelves and supports. The entire sterilization process takes 18 hours to ensure comprehensive, efficient sterilization;

B. UV Sterilization System (RWP)

  • The UV sterilization lamp at the rear top of the chamber periodically sterilizes the interior, effectively killing airborne bacteria in the circulating air and water vapor from the humidification tray or sloped bottom water tray, thereby preventing contamination during cell culture;

Independent Temperature / CO2 Concentration Monitoring System (Optional)

  • An optional external independent temperature and CO2 concentration monitoring system provides dual monitoring of chamber temperature and CO2 concentration;

Filter System

A. Microbial Filter

  • The CO2 inlet is fitted with a microbial filter. For particles with diameters ≥0.3μm, its filtration efficiency is up to 99.99%, effectively filtering bacteria and dust particles from the CO2 gas;

B. HEPA Filter

  • Gas quality inside a CO2 incubator is an important measure of the cell-culture environment. The HEPA filter effectively removes bacteria and dust particles from outside air, eliminating and preventing cross-contamination between outside air and the chamber to maintain sterile conditions. The HEPA filter is easily installed and removed without tools;

Safety Functions

  • High, Low and Overtemperature Alarms
  • Chamber Temperature Sensor Fault Alarm
  • Door temperature sensor fault alarm
  • Overtemperature Sensor Fault Alarm
  • High or low CO2 concentration alarm
  • Power Failure Alarm
  • Independent Temperature-Limit Alarm
  • Prolonged Door Opening Alarm
  • Disinfection / Sterilization Status Indication

Automatic Cylinder Changeover Device (Optional)

  • An optional automatic cylinder changeover device connects the CO2 inlet and N2/O2 inlet each to two cylinders, A/B. Automatic switching ensures continuous gas supply;

Tiered Access Management (Optional)

  • Multiple individual accounts can be assigned according to equipment-management requirements, with three controller access levels: administrator, operator and guest. The touchscreen supports Chinese and English input, allowing users to log in under their actual names. Detailed user-operation logs can also be queried for equipment operation, maintenance and audit trails;

Wireless Alarm System (SMS Alarm System) (Optional)

  • If the equipment user is away when a fault occurs, the system promptly collects fault signals and sends an SMS to the designated recipient’s mobile phone. This supports prompt fault correction and resumption of experiments, preventing accidental losses;

Technical Specifications

Technical Specification / Model BPN-40RHP(T) BPN-80RHP(T) BPN-150RHP(T) BPN-190RHP(T) BPN-240RHP(T) BPN-60RWP(T)
BPN-60RWP(90)
BPN-170RWP(T)
BPN-170RWP(90)
BPN-240RWP(T)
BPN-240RWP(90)
Supply Voltage AC220V/50HZ
Input Power 350W 500W 700W 750W 1000W 500W 700W 1000W
Heating Method Air-jacketed, microcomputer PID control Water-jacketed
Temperature Control Range RT+3~55°C
Ambient Operating Temperature +5~30°C
Temperature Uniformity ±0.3°C (@37°C)
Temperature Fluctuation ±0.1°C
CO2 Control Range 0~20%
CO2 Control Precision ±0.1% (infrared sensor)
CO2 Recovery Time (After door opening for 30 seconds, recovery to 5%) ≤3 minutes
O2 Concentration Range (Optional) 1%~90%
O2 Concentration Precision (Optional) ±0.1%
Temperature Recovery (After door opening for 30 seconds, recovery to 37°C) ≤8 minutes
Relative Humidity Natural evaporation ≥90% (optional digital relative-humidity display)
Capacity 40L 80L 155L 190L 233L 60L 170L 240L
Chamber Dimensions (mm)
W×D×H
400×286×350 400×450×500 480×530×610 520×530×690 600×630×670 380×290×550 530×460×720 600×520×780
Overall Dimensions (mm)
W×D×H
590×440×576 590×687×790 670×770×880 708×710×1030 790×840×940 534×530×790 684×700×960 754×760×1020
Shelves (Standard) 2 shelves 3 shelves 2 shelves 3 shelves
Disinfection Method 90°C high-temperature moist-heat sterilization UV disinfection + 90°C high-temperature moist-heat sterilization (90 series)

Optional Accessories:

1. RS485/232 interface and communication software
2. Dedicated CO2 pressure-reducing regulator
3. Humidity display system
4. Wireless alarm system (SMS alarm system)
5. Built-in printer 6. Tiered access management 7. Independent temperature/CO2 concentration monitoring system
Note: choose either RS485/232 or printer.

Note 1: All technical data are measured at an ambient temperature of 25°C;
Note 2: This series is sold only for non-medical applications;
Note 3: Three small doors are optional only for BPN-150/190/240RHP(T);
Note 4: BPN-60RWP(90)/170RWP(90)/240RWP(90) include 90°C high-temperature, high-humidity sterilization as standard;
Note 5: 3Q qualification and calibration services are optional.

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