Solid-Phase Extraction (SPE)(12,24)
Solid Phase Extraction (SPE) is widely used for the purification of small compound libraries synthesized in solution. Compared to the classic liquid-liquid extraction using the separatory funnel or the preparative HPLC purification it offers several advantages:
Reduced lab time
Easy manipulation
Higher concentration factor
No problem with the miscibility of solvent
Easy adaptable for very selective extraction
Avoids problems such as incomplete phase separations, less-than-quantitative recoveries and emulsion formation as encountered in liquid-liquid extractions
Independent valve to operate easily
6,12,24 groups to choice